FIB/SEM Processing of Biological Samples

Automated Force Volume Image Processing for Biological Samples

Atomic force microscopy (AFM) has now become a powerful technique for investigating on a molecular level, surface forces, nanomechanical properties of deformable particles, biomolecular interactions, kinetics, and dynamic processes. This paper specifically focuses on the analysis of AFM force curves collected on biological systems, in particular, bacteria. The goal is to provide fully automated...

Processing scarce biological samples for light and transmission electron microscopy.

Light microscopy (LM) and transmission electron microscopy (TEM) aim at understanding the relationship structure-function. With advances in biology, isolation and purification of scarce populations of cells or subcellular structures may not lead to enough biological material, for processing for LM and TEM. A protocol for preparation of scarce biological samples is presented. It is based on pre-...

Pre-PCR processing of samples.

Structural Study of Heterogeneous Biological Samples by Cryoelectron Microscopy and Image Processing

In living organisms, biological macromolecules are intrinsically flexible and naturally exist in multiple conformations. Modern electron microscopy, especially at liquid nitrogen temperatures (cryo-EM), is able to visualise biocomplexes in nearly native conditions and in multiple conformational states. The advances made during the last decade in electronic technology and software development ha...

Signal and Image Processing of Optical Coherence Tomography at 1310 Nm Wavelength for Non Biological Samples

OCT is a recently developed optical interferometric technique for non-invasive diagnostic medical imaging in vivo; the most sensitive optical imaging modality.OCT finds its application in ophthalmology, blood flow estimation and cancer diagnosis along with many non biomedical applications. The main advantage of OCT is its high resolution which is in μm range and depth of penetration in mm range...